Dynamics of glutamine synthetase expression in hepatic ischemia-reperfusion injury: Implications for therapeutic interventions

Figure 5 Distribution of glutamine synthetase in the liver after 24 hours of ischemia-reperfusion. A: Following 1 hour of ischemia, C57BL/6J mice resumed perfusion, and liver tissues were collected 24 hours later. Harvested liver tissues were processed for hematoxylin and eosin staining. Scale bar: 100 μm; B: Liver tissues were processed for immunofluorescence staining using glutamine synthetase (green). Scale bar: 100 μm. Representative images are displayed, with six mice in each group. The experiment was repeated at least three times to ensure reproducibility and reliability of the results. Representative images are displayed, with six mice in each group. The experiment was repeated at least three times to ensure reproducibility and reliability of the results. PV: Portal vessels; CV: Central vessels; HE: Hematoxylin and eosin; IR: Ischemia-reperfusion.