Yinhuang granule alleviates carbon tetrachloride-induced liver fibrosis in mice and its mechanism

Figure 6 Yinhuang granule induced mitochondrial biogenesis in carbon tetrachloride-treated mice. A: Liver mitochondrial DNA (mtDNA) copy numbers (n = 5); B: Liver adenosine triphosphate level (n = 5); C: The expression of liver peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1α) and nuclear respiratory factor 1 (NRF1) was detected by Western blot, and b-actin was used as a loading control. The results represent three independent experiments; D: The protein bands of PGC1a and NRF1 were normalized to basal b-actin expression (n = 3). Data were expressed as mean ± SEM. ^aP < 0.05, ^bP < 0.01 vs control vehicle; ^cP < 0.05, ^dP < 0.01 vs carbon tetrachloride vehicle. ATP: Adenosine triphosphate; CCl₄: Carbon tetrachloride; YHG: Yinhuang granule; PGC1α: Proliferator-activated receptor gamma coactivator 1 alpha; NRF1: Nuclear respiratory factor 1.