Yinhuang granule alleviates carbon tetrachloride-induced liver fibrosis in mice and its mechanism

Figure 5 Yinhuang granule induced the activation of hepatic nuclear factor erythroid 2-related factor 2 antioxidant signaling pathway in carbon tetrachloride-treated mice. A: The expression of liver hepatic nuclear factor erythroid 2-related factor 2 (Nrf2) was detected by Western blot, and b-actin and Lamin B1 were used as loading controls. The results represent at least three independent experiments; B: The protein bands of Nrf2 were normalized to basal b-actin or Lamin B1 expression (n = 3-4); C: Hepatic mRNA expression of NAD(P)H:quinone oxidoreductase-1 (NQO1), glutamate-cysteine ligase (GCLC) and modifier subunit of glutamate-cysteine ligase (GCLM) (n = 3); D: The expression of liver NQO1, GCLC and GCLM was detected by Western blot, and b-actin was used as a loading control. The results represent at least three independent experiments; E: The protein bands of NQO1, GCLC and GCLM were normalized to basal b-actin expression (n = 3-4). Data were expressed as mean ± SEM. ^aP < 0.05 vs control vehicle; ^cP < 0.05 vs carbon tetrachloride vehicle. CCl₄: Carbon tetrachloride; YHG: Yinhuang granule; Nrf2: Nuclear factor erythroid 2-related factor 2; NQO1: NAD(P)H:quinone oxidoreductase 1; GCLC: Glutamate-cysteine ligase; GCLM: Glutamate-cysteine ligase.