Yinhuang granule alleviates carbon tetrachloride-induced liver fibrosis in mice and its mechanism

doi:10.4254/wjh.v16.i2.264

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Feb 27, 2024 (publication date) through Oct 2, 2024

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World Journal of Hepatology

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1948-5182

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Hepatol. Feb 27, 2024; 16(2): 264-278
Published online Feb 27, 2024. doi: 10.4254/wjh.v16.i2.264

Table 2 List of Primers for real-time polymerase chain reaction

Target	Primer	Sequence (5'-3')
Col1a1	FP	TGACTGGAAGAGCGGAGAGT
Col1a1	RP	GACGGCTGAGTAGGGAACAC
Col3a1	FP	ATGGGTTTCCCTGGTCCTAA
Col3a1	RP	TGCCTTGTAATCCTTGTGGA
Fn1	FP	AGAACCAGAGGAGGCACAAG
Fn1	RP	CCGTGTAAGGGTCAAAGCAT
Tgfb1	FP	TGCCCTCTACAACCAACACA
Tgfb1	RP	GTTGGACAACTGCTCCACCT
Acta2	FP	GGGAGTAATGGTTGGAATGG
Acta2	RP	GGTGATGATGCCGTGTTCTA
TNFα	FP	AGGCACTCCCCCAAAAGAT
TNFα	RP	CAGTAGACAGAAGAGCGTGGTG
IL-1β	FP	AGTTGACGGACCCCAAAAG
IL-1β	RP	CTTCTCCACAGCCACAATGA
IL-6	FP	ACAAAGCCAGAGTCCTTCAGAGAG
IL-6	RP	TTGGATGGTCTTGGTCCTTAGCC
iNOS	FP	CAGGCGGTGCCTATGTCTC
iNOS	RP	CAGCTGGGCTGTACAAACCTT
Nqo1	FP	CTCGTGGAGACGCTTTACAT
Nqo1	RP	CGTTTCTTCCATCCTTCCAG
Gclc	FP	CGGAGGAACGATGTCTGAGT
Gclc	RP	CTGGGGAATGAAGTGATGGT
Gclm	FP	CAATGACCCGAAAGAACTGC
Gclm	RP	CAATGACCCGAAAGAACTGC
Actin	FP	TTCGTTGCCGGTCCACACCC
Actin	RP	GCTTTGCACATGCCGGAGCC
18s	FP	CGCGGTTCTATTTTGTTGGT
18s	RP	AGTCGGCATCGTTTATGGTC
ND1	FP	CTAGCAGAAACAAACCGGGC
ND1	RP	CCGGCTGCGTATTCTACGTT

FP: Forward primer; RP: Reverse primer; TNF: Tumour necrosis factor; IL: Interleukin; iNOS: Inducible nitric oxide synthase.

Citation: Ouyang H, Miao H, Li Z, Wu D, Gao SC, Dai YY, Gao XD, Chai HS, Hu WY, Zhu JF. Yinhuang granule alleviates carbon tetrachloride-induced liver fibrosis in mice and its mechanism. World J Hepatol 2024; 16(2): 264-278
URL: https://www.wjgnet.com/1948-5182/full/v16/i2/264.htm
DOI: https://dx.doi.org/10.4254/wjh.v16.i2.264