Subcellular distribution of prohibitin 1 in rat liver during liver regeneration and its cellular implication

Figure 1 Altered expression of prohibitin 1 mRNA and protein during liver regeneration. A: Altered expression of prohibitin 1 (Phb1) mRNA during liver regeneration (LR). Rat livers were homogenized and harvested with TRIzol reagent. Real-time polymerase chain reaction (PCR) amplifications were performed, standardized by the amounts of β-actin. The real-time PCR signals were analyzed with LightCycler 3.5 software; B: Altered expression of Phb1 protein during LR. Equal amounts of protein from each sample were separated on 12% SDS-PAGE and immunoblotted with antibodies against Phb1 and control protein, β-actin. On the left are the western blotting results, and on the right is a gray analysis of the results showing the fold changes of Phb1 expression in PH groups in comparison with SH groups, standardized by the amounts of β-actin. All grouping of gels/blots were cropped from different parts of the same gel. PH: 70% PHx test groups; PHx: Partial hepatectomy; SH: Sham-operation control groups;. Results are from three independent experiments and data are represented as means ± SD. ^aP < 0.05, versus control group, significant difference.