Development of the nervous system in mouse liver

Figure 7 Immunohistochemical analysis of the portal tract at postnatal day 7. A-C: Center of the liver. Distribution of protein gene product 9.5 (PGP9.5)-positive nerve fibers were mainly observed around the hepatic artery (C, thick arrow) and portal vein branches, with less association with bile ducts (B and C); D-F: Periphery of the liver. SRY-related high mobility group-box gene 9-positive cells constructed cytokeratin 19 (CK19)-positive bile ducts not only in the center but also in the periphery. The number of PGP9.5-positive nerve fibers increased in the portal tracts even in the periphery. Scale bar = 200 μm. PV: Portal vein; HA: Hepatic artery; BD: Bile duct; HE: Hematoxylin-eosin; CK19: Cytokeratin 19; SOX9: SRY-related high mobility group-box gene 9; PGP9.5: Protein gene product 9.5.