Development of the nervous system in mouse liver

Figure 5 Immunohistochemical analysis of the center of the liver at embryonic day 16. 5 and embryonic day 17.5. A-D: Embryonic day 16.5; E and F: E17.5; A: Protein gene product 9.5 (PGP9.5)-positive bodies were found in the periportal tissue; B: Cytokeratin 19 (CK19) was partly positive in SRY-related high mobility group-box gene 9 (SOX9)-positive structures; C and D: SOX9-positive cells partly from double-layered cylindrical structures called ductal plates (arrowhead) and partly form ductal structures; C: The arrow indicates the hilar bile duct; E: Jagged-1 was mainly expressed in the periportal tissue (thick arrow); F: Hepatocyte nuclear factor 4α was expressed in the liver parenchyma but not in the Jagged-1-positive periportal tissue (thick arrow). Scale bars = 200 μm. CK19: Cytokeratin 19; SOX9: SRY-related high mobility group-box gene 9; PGP9.5: Protein gene product 9.5; HNF4α: Hepatocyte nuclear factor 4α.