Long-term and non-invasive in vivo tracking of DiD dye-labeled human hepatic progenitors in chronic liver disease models

Figure 2 Flow cytometry characterization of epithelial cell adhesion molecule expressing fetal hepatic progenitors, and DiD labeling of magnetically activated cell sorting enriched epithelial cell adhesion molecule-positive cells. A and B: Total fetal liver progenitors were stained with epithelial cell adhesion molecule (EpCAM)-fluorescein isothiocyanate (FITC) antibody and the representative dot plots of (A) unstained and EpCAM (CD326) stained cells and (B) overlay histogram; C: Colocalization of pan CK and c-Met with EpCAM-FITC expression (Scale bar = 10 μm); D: Overlay histogram of magnetically sorted EpCAM-positive cells labeled with DiD; E: Near-infrared fluorescence imaging and X-ray overlay image of DiD-labeled and unlabeled EpCAM-positive cells in the tube. EpCAM: Epithelial cell adhesion molecule; NIR: Near-infrared; DAPI: 4',6-diamidino-2-phenylindole; MACS: Magneticallyactivated cell sorting.