Long-term and non-invasive in vivo tracking of DiD dye-labeled human hepatic progenitors in chronic liver disease models

Figure 1 Schematic presentation of the study. A: Total fetal liver cells were enriched for EpCAM-positive cells by magnetically activated cell sorting; B: Cells were labeled with DiD and visualized in the tube by near-infrared imaging as red fluorescence; C: Cells were intra-hepatically transplanted into injured livers of severe combined immunodeficiency mice; D: In vivo imaging of live animals was performed at specified time points; E: Ex vivo imaging of liver confirmed the retention of DiD-labeled transplanted cells in the liver. EpCAM: Epithelial cell adhesion molecule; SCID: Severe combined immunodeficiency; MACS: Magnetic activated cell sorting.