BIR repeat-containing ubiquitin conjugating enzyme (BRUCE) regulation of β-catenin signaling in the progression of drug-induced hepatic fibrosis and carcinogenesis

Figure 5 BRUCE deficiency increases nuclear β-catenin and activity in hepatocellular carcinoma livers. A and B: Immunohistochemistry of β-catenin in liver tumors after 14 mo of DEN-exposure which is quantified in; C: RT-PCR analysis of β-catenin in liver tumors after 14 mo post-DEN revealed an increase in mRNA levels in BRUCE knockout livers compared to control; D and E: Graphical representation of western blot analysis of β-catenin in liver tumors after 14 mo of DEN-exposure and cyclin D1, a downstream target of β-catenin. ^aP < 0.05. HCC: Hepatocellular carcinoma; LKO: Liver-specific knockout; CTRL: Control.