Promotive action of 2-acetylaminofluorene on hepatic precancerous lesions initiated by diethylnitrosamine in rats: Molecular study

Table 2 Effect of diethylnitrosamine and 2-acetylaminofluorene on alpha-fetoprotein and liver function

	AFP	ALT	Total bilirubin	Direct bilirubin	Albumin
Naïve	22.8 ± 1.13	33.3 ± 6.83	0.30 ± 0.18	0.27 ± 0.14	3.77 ± 0.23
Week 10
DEN	89.2 ± 28.8d	63.0 ± 27.5d	1.44 ± 0.45d	1.03 ± 0.14d	2.49 ± 0.15d
DEN + 100 AAF	116 ± 52.1d	78.3 ± 17.8d	2.07 ± 0.44d,e	1.40 ± 0.39d	2.83 ± 0.19d
DEN + 200 AAF	223 ± 124b,d	82.7 ± 12.7d	2.73 ± 0.23a,d,e	1.67 ± 0.19a,d,e	2.73 ± 0.14d
DEN + 300 AAF	305 ± 126d,e	98.0 ± 10.7d,e	3.13 ± 0.36b,d,e	2.13 ± 0.61d,e	3.15 ± 0.38c,d,e
Week 16
DEN	159 ± 32.2d	94.1 ± 6.4d	2.13 ± 0.55d	1.60 ± 0.39d	2.0 ± 0.62d
DEN + 100 AAF	290 ± 241d	104 ± 31.9d	2.23 ± 0.36d	2.25 ± 0.63d	2.57 ± 0.37d
DEN + 200 AAF	815 ± 143a,d,f	128 ± 36.9d	4.10 ± 0.39a,d,f	2.53 ± 0.63a,d,f	2.17 ± 0.29d
DEN + 300 AAF	1059 ± 360b,d,f	210 ± 63.2b,c,d,f	4.47 ± 0.99b,d,f	3.10 ± 0.39d,f	2.13 ± 0.67b,c,d,f

Values are mean ± SD; number of animals = 6 rats/each group.

^aP < 0.05 when DEN + 200 acetylaminofluorene (AAF) is compared to the DEN + 100 AAF.

^bP < 0.05 when DEN + 300 AAF is compared to the DEN + 100 AAF.

^cP < 0.05 when DEN + 300 AAF is compared to the DEN + 200 AAF.

^dP < 0.05 compared to the naïve group.

^eP < 0.05 compared to the diethylnitrosamine (DEN) group at week 10 group.

^fP < 0.05 compared to the DEN at week 16 group. One-way ANOVA followed by Tukey's multiple comparison test. DEN: Diethylnitrosamine; 2-AAF: 2-Acetylaminofluorene; AFP: Alpha-fetoprotein; ALT: Alanine aminotransferase.