Direct modulation of hepatocyte hepcidin signaling by iron

Table 1 Primer list of the genes analyzed by quantitative real-time polymerase chain reaction

Gene	Primer sequence
human β2-mg	forward: 5’-tga ctt tgt cac agc cca aga ta-3’
	reverse: 5’-aat cca aat gcg gca tct tc-3’
	probe: FAM-tga tgc tgc tta cat gtc tcg atc cca-TAM
human GAPDH	forward: 5′-gaa ggt gaa ggt cgg agt-3’
	reverse: 5′-gaa gat ggt gat ggg att tc-3’
	probe: FAM-caa gct tcc cgt tct cag cc-TAM
human hepcidin	forward 5′-cag gac aga gct gga gcc a-3′
	reverse: 5′-gca gca cat ccc aca ctt tg-3′
	probe: FAM-ctg ctg cgg ctg ctg tca tcg a-TAM
human SMAD6	forward: 5′-tgc aac ccc tac cac ttc a-3′
	reverse: 5′-cga gga gac agc cga gag t-3′
	probe UPL # 10 (Roche)
mouse HPRT	forward: 5′-ggt cca ttc cta tga ctg tag att tt-3′
	reverse: 5′-caa tca aga cgt tct ttc cag tt-3′
	probe UPL # 22 (Roche)