N-acetylcysteine and glycyrrhizin combination: Benefit outcome in a murine model of acetaminophen-induced liver failure

Figure 10 Protective effects of the N-acetylcysteine/glycyrrhizin combination do not result from inhibition of hepatic expression of CYP2E1. A: CYP2E1 mRNA expression was assessed by RT-qPCR in liver extract of vehicle-treated mice and mice sacrificed 2, 6, 12 or 24 h after acetaminophen (APAP, 500 mg/kg) injection (5 mice in each group); B: CYP2E1 mRNA expression was assessed by RT-qPCR in liver extract of mice treated with glycyrrhizin (GL, 200 mg/kg), N-acetylcysteine (NAC, 150 mg/kg), or NAC/GL, at the same time of APAP (10 mice in each group). Mice were scarified 12 h after APAP administration. The relative expression of the gene of interest was calculated using the Pfaffi method. ^cP < 0.05, ^dP < 0.01, ^eP < 0.001 vs APAP. Experiments were reproduced three times.