N-acetylcysteine and glycyrrhizin combination: Benefit outcome in a murine model of acetaminophen-induced liver failure

Figure 7 Delayed administration of N-acetylcysteine/glycyrrhizin combination reduced high mobility group box 1 release as well as glycyrrhizin alone. A: High mobility group box 1 (HMGB1) levels were measured in the plasma of vehicle-treated mice and mice treated with N-acetylcysteine (NAC, 150 mg/kg), glycyrrhizin (GL, 200 mg/kg) or NAC/GL, 2 h or 6 h after acetaminophen (APAP) injection (10 mice in each group). Mice were scarified 12 h after APAP administration; B: Quantification of nuclear expression of HMGB1 in the same groups of mice; C: Representative hematoxylin and eosin (H&E) and HMGB1-stained images (magnification × 200) of murine liver 12 h after APAP challenge in the same group of mice. Results are expressed as mean ± standard error. ^cP < 0.05, ^dP < 0.01, ^eP < 0.001 vs APAP. Experiments were reproduced three times.