N-acetylcysteine and glycyrrhizin combination: Benefit outcome in a murine model of acetaminophen-induced liver failure

Figure 4 Administration of N-acetylcysteine/glycyrrhizin combination, at the same time as acetaminophen, reduced the severity of liver injury as well as glycyrrhizin or N-acetylcysteine alone. A: Alanine aminotransferase (ALT) levels were measured in the plasma of vehicle-treated mice and mice treated with N-acetylcysteine (NAC, 150 mg/kg), glycyrrhizin (GL, 200 mg/kg) or NAC/GL, 2 h or 6 h after acetaminophen (APAP) injection (10 mice in each group); B: Aspartate aminotransferase (AST) levels were measured in the plasma of vehicle-treated mice and mice treated with NAC (150 mg/kg), GL (200 mg/kg) or NAC/GL, 2 h or 6 h after APAP injection (10 mice in each group); C: Lactate dehydrogenase (LDH) levels were measured in the plasma of vehicle-treated mice and mice treated with NAC (150 mg/kg), GL (200 mg/kg) or NAC/GL, 2 h or 6 h after APAP injection (10 mice in each group); D: Liver necrosis at 12 h after APAP challenge was scored in the same group of mice; E: Representative hematoxylin and eosin (H&E) and high mobility group box 1 (HMGB1)-stained images (magnification × 200) of murine liver 12 h after of murine liver 12 h after vehicle or APAP challenge; F: HMGB1 levels were measured in the same group of mice; G: Quantification of nuclear expression of HMGB1 in the same groups of mice. Mice were scarified 12 h after APAP administration. Results are expressed as mean ± standard error. ^cP < 0.05, ^dP < 0.01, ^eP < 0.001 vs APAP. Experiments were reproduced three times.