N-acetylcysteine and glycyrrhizin combination: Benefit outcome in a murine model of acetaminophen-induced liver failure

Figure 3 Administration of N-acetylcysteine, at the same time as acetaminophen, reduced the severity of liver injury. A: Alanine aminotransferase (ALT) levels were measured after 12 h in the plasma of mice treated by vehicle (phosphate-buffered saline; PBS) or N-acetylcysteine (NAC, 150 mg/kg) at the time of acetaminophen (APAP, 500 mg/kg) injection (n = 10); B: Aspartate aminotransferase (AST) levels were measured after 12 h in the plasma of mice treated by vehicle (PBS) or NAC (150 mg/kg) at the time of APAP (500 mg/kg) injection (n = 10); C: Lactate dehydrogenase (LDH) levels were measured after 12 h in the plasma of mice treated by vehicle (PBS) or NAC (150 mg/kg) at the time of APAP (500 mg/kg) injection (n = 10); D: Liver necrosis was scored in the same group of mice; E: Representative hematoxylin and eosin (H&E) and high mobility group box 1 (HMGB1)-stained images (magnification × 200) of murine liver 12 h after APAP challenge in the same group of mice; F: HMGB1 levels were measured in the same group of mice; G: Quantification of nuclear expression of HMGB1 in the same groups of mice; Results are expressed as mean ± standard error. ^cP < 0.05, ^dP < 0.01, ^eP < 0.001 vs APAP. Experiments were reproduced three times.