Update on diagnosis and management of sepsis in cirrhosis: Current advances

Table 2 Transcriptomics based micro assays for diagnosis of sepsis[57]

Assay name (manufacturer)	Technique/sample volume	Turn-around time	Highest noted sensitivity and specificity	Detection
SeptiFast (Roche)	Real-time PCR/1.5 mL	4 h to 6 h	83%/95%	> 16 bacteria, Candida and Aspergillus fumigatus
SeptiTest (Molzyme)	Universal PCR/1 mL	8 h to 10 h	87%/96%	> 345 bacteria and 13 fungi
SeptiCyte (ImmuneExpress)	RT-qPCR with machine learning/2.5 mL	1 h to 6 h	-/ 95% (discriminates SIRS from sepsis)	All pathogens
Iridica Plex ID (Abbott)	Multiplex broad range PCR/5 mL	6 h	83%/94%	780 bacteria and Candida
MinION (Oxford Nanopore)	Nanopore sequencing/10 ng DNA	4 h to 6 h	-/100%	Few viruses and bacteria currently
U-dHRM (UCSD, United States)	Digital PCR/1 mL	3 h	-/99.9%	37 bacteria
LAMP Tech	Loop mediated isothermal amplification/30 µL	1 h	-/100%	1 pathogen per sample
Integrated droplet digital detection tech (Velox Biosystems)	DNA-zyme base sensor droplet microencapsulation 3D particle analysis	1 h to 4 h	-	1 pathogen per sample

3D: Three-dimensional; PCR: Polymerase chain reaction; RT: Reverse transcriptase; SIRS: Systemic inflammatory response syndrome.