Ipragliflozin-induced improvement of liver steatosis in obese mice may involve sirtuin signaling

Figure 6 Hepatic and renal mRNA expression of sodium glucose cotransporter 2 and phosphorylation of AMP-activated protein kinase in whole livers of obese mice treated with or without ipragliflozin and their lean littermates. A: Reverse transcription-polymerase chain reaction analysis of SGLT2 expression in mouse livers and kidneys; B: Representative western blot showing the levels of phosphorylated phospho-AMP-activated protein kinase (p-AMPK) at the end of the 4-wk period of ipragliflozin treatment; C: The expression of p-AMPK protein was normalized to that of t-AMPK protein. ^aP < 0.05 vs the ob/ob group. Ipra: Ipragliflozin; p-AMPK: Phospho AMP-activated protein kinase; SGLT2: Sodium glucose cotransporter 2; t-AMPK: Total AMP-activated protein kinase. n = 8.