Autophagy related protein 9A increase in hepatitis B virus-associated hepatocellular carcinoma and the role in apoptosis

Figure 2 Silencing of autophagy related protein 9A by RNA interference and detection of hepatitis B virus DNA level. A: The western blot method was applied to analyze autophagy related protein 9 (ATG9A) protein levels against a mock treatment (control) and ATG9A knockdown (shATG9A) HepG2.2.15 cells; B: Quantitation of hepatitis B virus (HBV) DNA by real-time PCR. Total purified DNA from mock treatment (mock) and ATG9A knockdown (shATG9A) in HepG2.2.15 cells was amplified using preS1 specific primers. Hepatitis B virus preS1 plasmid was used as standard copy number. Data is shown as mean ± standard error of the four independent experiments. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.