Pivotal role of long non-coding ribonucleic acid-X-inactive specific transcript in regulating immune checkpoint programmed death ligand 1 through a shared pathway between miR-194-5p and miR-155-5p in hepatocellular carcinoma

Figure 8 Impact of oleuropein on the expression profile of the study key players. The expression pattern of programmed death ligand 1 (PD-L1), micro ribonucleic acids 194-5p and 155-5p as well as long non-coding ribonucleic acids X-inactive specific transcript (XIST) and MALAT-1 were assessed following oleuropein treatment. Values for micro ribonucleic acids were normalized to RNU6B while that for PD-L1, XIST and MALAT-1 were normalized to B2M. Enhanced expression of miR-194-5p was observed following oleuropein treatment (P = 0.0022). However, PD-L1, XIST and miR-155-5p expression pattern were down regulated, (P = 0.0011), (P = 0.0020) and (P = 0.0001), respectively. Oleuropein did not show any significant impact on MALAT-1 expression profile. ^bP < 0.01 and ^cP < 0.001.