Pivotal role of long non-coding ribonucleic acid-X-inactive specific transcript in regulating immune checkpoint programmed death ligand 1 through a shared pathway between miR-194-5p and miR-155-5p in hepatocellular carcinoma

Figure 3 Transfection efficiency in Huh-7 cells with miR194-5p and miR-155-5p oligonucleotides. Using quantified real-time-polymerase chain reaction, the transfection efficiency was determined in both mimicked and mock cells 48 h post-transfection for each of the respective mi-ribonucleic acids. MiR-194-5p and miR-155-5p were normalized to RNU6B as an endogenous control. A: Mimicking of miR-194-5p; and B: miR-155-5p resulted in an increase in each of the respective miRNAs, (P = 0.0026) and (P < 0.0001), respectively. The expression levels were compared with the unpaired Student’s t-test. ^bP < 0.01 and ^cP < 0.001.