Cyclin-dependent kinase inhibitors p21 and p27 function as critical regulators of liver regeneration following 90% hepatectomy in the rat

Figure 3 S-phase progression is markedly delayed after extended hepatectomy. Animals were sacrificed at the indicated time points after 70% [hepatectomy (pHx)] or 90% [extended hepatectomy (eHx)], and remnant liver tissues were harvested for immunofluorescence analysis. A: Bromodeoxyuridine (BrdU) incorporation; B: Phospho-histone H3 Labeling of mitotic cells. Cell counting was performed in 10 random microscopic fields on 6 rats in the eHx group and 3 rats in pHx; C: Mean liver to body weight ratios after pHx and eHx. eHx: Extended hepatectomy; pHx: 70% hepatectomy.