Cyclin-dependent kinase inhibitors p21 and p27 function as critical regulators of liver regeneration following 90% hepatectomy in the rat

Figure 1 Survival rates and post-operative liver injury after enlarged liver resection. Male Wistar rats were subjected to 70% [hepatectomy (pHx)] or 90% [extended hepatectomy (eHx)] followed by harvest of blood samples and the remnant liver tissues at the indicated time points after surgery. A: Kaplan-Meier survival plots; B: Serum levels of alanine aminotransferase (ALT); C: Serum levels of aspartate aminotransferase (AST); D: Conjugated bilirubin from hours 24 to 168 post-eHx (n = 45); E: Representative liver images stained with hematoxylin, eosin, and alcian blue at the indicated time points post-eHx; black arrowheads indicate mitotic figures, and white arrowheads point to globular red hyaline material. Scale bar: 50 μM. Sham: Laparotomy control group.