Prolonged high-fat-diet feeding promotes non-alcoholic fatty liver disease and alters gut microbiota in mice

Figure 5 Endoplasmic reticulum stress signaling in the liver tissue of mice subjected to prolonged high-fat diet-feeding. A: Western blot densitometry analysis of binding immunoglobulin; B: Western blot densitometry analysis of phosphorylated inositol-requiring enzyme-1; C: Western blot densitometry analysis of X-box-binding protein-1; D: Western blot densitometry analysis of phosphorylated eukaryotic translation initiation factor 2α; E: Western blot densitometry analysis of phosphorylated c-Jun-N-terminal kinase; F: Western blot densitometry analysis of C/EBP-homologous protein. Data are expressed as mean ± SE. n = 9-10 mice per group. ^aSignificantly different from Young-LFD (P < 0.05). ^bSignificantly different from Old-LFD (P < 0.05). Bip: Binding immunoglobulin; IRE1α: Inositol-requiring enzyme-1; XBP1s: X-box-binding protein-1; EIF2α: Eukaryotic translation initiation factor 2α; Jnk: Jun-N-terminal kinas; CHOP: C/EBP-homologous protein; LFD: Low-fat diet.