Experimental bio-artificial liver: Importance of the architectural design on ammonia detoxification performance

Table 1 Composition of the different Krebs-Henseleit solution used

Components	KHB solution	KHA solution
NaCl	114 mmol/L	114 mmol/L
KH2PO4	1.2 mmol/L	1.2 mmol/L
KCl	4.8 mmol/L	4.8 mmol/L
MgSO4	1.2 mmol/L	1.2 mmol/L
CaCl2	1.5 mmol/L	1.5 mmol/L
HEPES	10 mmol/L	10 mmol/L
NaHCO3	25 mmol/L	25 mmol/L
Glucose	25 mmol/L	25 mmol/L
Allopurinol	1 mmol/L	1 mmol/L
Fructose	5 mmol/L	5 mmol/L
Glycine	-	3 mmol/L
Adenosine	-	10 µmol/L
Ornithine	-	6 mmol/L
Sodium lactate	-	10 mmol/L
pH	7.4	7.4
mOsm/kg H2O	293 ± 6 (n = 10)	328 ± 5 (n = 10)

HEPES: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; mOsm/kg H₂O: Osmolality; KHB: KH-Base solution; KHA: KH-ammonia solution.