Bioengineered humanized livers as better three-dimensional drug testing model system

Figure 3 Characterization of acellularized liver scaffold. A: Ultra-structural characterization of acellularized xenogenic liver showing acellularity in the scaffold with preserved three-dimensional microanatomy of the portal tract surrounded by lobular structures. The parenchymal spaces were found enriched with connective tissue fibres arranged with honeycomb-like structures which are an exceptionally preserved anatomy of connective tissues which structures the hepatocyte-free spaces; B: Methylene blue dye infusion showing intact vasculature post-acellularization; Residual DNA content in liver perfusate (C) and in liver tissues (D) before (black bar) and after acellularization (red bar) (^bP < 0.0001); E: Mixed tensile strength; F: Mixed suture retention strength; G: Mixed comprehensive strength plots showing higher degree of retention of mechanical properties post-acellularization.