Partial blockage of hepatocyte maturation in hepatoma-derived growth factor transgenic mice

Figure 2 Genomic integration and mRNA expression of the HDGF-transgene. A: Southern blot analysis with HDGF cDNA probe. Genomic DNA was isolated from individual mouse tails and Southern blot analysis was performed according to standard methods. The bands representing endogenous HDGF and transgenes are shown. Genomic DNA of a transgenic mice (line number 21: Tg-21) contains high copy numbers of the transgene. Genomic DNA of the other transgenic mice (line number 48: Tg-48) contains low copy numbers of the transgene; B: Northern blot analysis with HDGF cDNA probe. Total RNA was isolated from liver tissues of both transgenic (Tg) and wild-type (WT) mice. Twenty micrograms of total RNA extracted was loaded and hybridized with mouse cDNA of the HDGF-specific sequence. HDGF expression was high in the liver of the Tg-48 mouse. The expression level of HDGF in the liver of Tg-21 was almost equal to the level expressed in the control liver. Ribosomal RNA of 18S is shown in the lower panel.