Murine hepatocellular carcinoma derived stem cells reveal epithelial-to-mesenchymal plasticity

Figure 4 Three-dimensional spheres have higher expression of mesenchymal markers. qRT-PCR analysis revealed upregulation of A: Vimentin and B: Fibronectin in Hepa 1-6 3-dimensional spheres compared with Hepa 1-6 grown as adherent cells. qRT-PCR analysis revealed higher expression of C: Vimentin and D: Fibronectin in AML12 3-dimensional spheres compared with AML12 grown as adherent cells. Values are mean ± SEM of three experiments in triplicate (^aP < 0.05, ^bP < 0.01, ^eP < 0.001). qRT-PCR: Quantitative reverse transcriptase-PCR; ActB: Beta-Actin.