Basic Study
Copyright ©The Author(s) 2017.
World J Stem Cells. Sep 26, 2017; 9(9): 159-168
Published online Sep 26, 2017. doi: 10.4252/wjsc.v9.i9.159
Figure 2
Figure 2 Three-dimensional spheres expressed high stemness and cell surface cancer stem cell markers. qRT-PCR analysis revealed higher expression of A: Klf4, B: Bmi1 and C: Cd44 in Hepa 1-6 3-dimensional spheres compared with Hepa 1-6 grown as adherent cells. qRT-PCR analysis revealed higher expression of D: Klf4, E: Bmi1 and F: Cd44 in AML12 3-dimensional spheres compared with AML12 grown as adherent cells. Values are mean ± SEM of three experiments in triplicate (aP < 0.05, bP < 0.01, eP < 0.001). qRT-PCR: Quantitative reverse transcriptase-PCR; ActB: Beta-Actin.