Next-generation sequencing traces human induced pluripotent stem cell lines clonally generated from heterogeneous cancer tissue

doi:10.4252/wjsc.v9.i5.77

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World Journal of Stem Cells

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World J Stem Cells. May 26, 2017; 9(5): 77-88
Published online May 26, 2017. doi: 10.4252/wjsc.v9.i5.77

Table 7 Allelic depth of single-nucleotide variants among the matched adjacent non-cancerous tissue, the starting cancer tissue, and the cancer tissue-derived induced pluripotent stem cell lines

	Allelic depth of SNVs
SNV No.	NCC1	CC1	CC1-1	CC1-2	CC1-7	CC1-8	CC1-9	CC1-11	CC1-12	CC1-17	CC1-18	CC1-25
1	250\|0	246\|0	232\|0	250\|0	250\|0	250\|0	250\|0	248\|0	250\|0	250\|0	129\|121	250\|0
2	249\|0	240\|0	240\|0	248\|0	248\|1	250\|0	129\|121	248\|0	242\|0	250\|0	250\|0	244\|0
3	246\|0	247\|2	249\|0	238\|1	246\|0	248\|0	233\|0	241\|0	238\|1	241\|0	245\|0	132\|106
4	250\|0	239\|0	243\|0	248\|0	245\|0	120\|129	250\|0	236\|0	250\|0	250\|0	250\|0	249\|0
5	216\|0	150\|0	75\|79	189\|0	184\|0	180\|0	200\|1	131\|0	176\|0	221\|0	207\|0	179\|0
6	249\|0	238\|0	250\|0	132\|114	250\|0	250\|0	242\|0	248\|0	248\|0	250\|0	250\|0	249\|0
7	250\|0	248\|0	250\|0	250\|0	245\|0	246\|0	245\|0	135\|111	249\|0	250\|0	250\|0	246\|1
8	233\|0	240\|1	243\|0	250\|0	245\|0	242\|0	247\|0	248\|0	132\|113	240\|1	247\|0	241\|0
9	249\|0	246\|2	250\|0	250\|0	249\|0	220\|30	244\|0	249\|0	250\|0	249\|1	250\|0	249\|0
10	247\|0	177\|0	188\|0	119\|121	198\|0	244\|0	241\|0	176\|0	221\|0	224\|0	249\|0	174\|0
11	246\|1	172\|0	181\|0	208\|0	209\|0	198\|0	189\|0	175\|0	244\|0	182\|0	233\|0	95\|87
12	249\|1	195\|54	241\|0	249\|0	249\|0	249\|1	249\|0	250\|0	249\|0	250\|0	249\|1	250\|0
13	137\|0	91\|10	79\|0	131\|0	102\|0	103\|0	103\|0	83\|0	106\|0	111\|0	142\|0	90\|0

NCC1: The matched adjacent non-cancerous tissue; CC1: The starting cancer tissue of the induced pluripotent stem cell lines; CC1-1 to CC1-25: Each induced pluripotent stem cell line; SNV: Single-nucleotide variant.

Citation: Ishikawa T. Next-generation sequencing traces human induced pluripotent stem cell lines clonally generated from heterogeneous cancer tissue. World J Stem Cells 2017; 9(5): 77-88
URL: https://www.wjgnet.com/1948-0210/full/v9/i5/77.htm
DOI: https://dx.doi.org/10.4252/wjsc.v9.i5.77

	Allelic depth of SNVs
SNV No.	NCC1	CC1	CC1-1	CC1-2	CC1-7	CC1-8	CC1-9	CC1-11	CC1-12	CC1-17	CC1-18	CC1-25
1	250\|0	246\|0	232\|0	250\|0	250\|0	250\|0	250\|0	248\|0	250\|0	250\|0	129\|121	250\|0
2	249\|0	240\|0	240\|0	248\|0	248\|1	250\|0	129\|121	248\|0	242\|0	250\|0	250\|0	244\|0
3	246\|0	247\|2	249\|0	238\|1	246\|0	248\|0	233\|0	241\|0	238\|1	241\|0	245\|0	132\|106
4	250\|0	239\|0	243\|0	248\|0	245\|0	120\|129	250\|0	236\|0	250\|0	250\|0	250\|0	249\|0
5	216\|0	150\|0	75\|79	189\|0	184\|0	180\|0	200\|1	131\|0	176\|0	221\|0	207\|0	179\|0
6	249\|0	238\|0	250\|0	132\|114	250\|0	250\|0	242\|0	248\|0	248\|0	250\|0	250\|0	249\|0
7	250\|0	248\|0	250\|0	250\|0	245\|0	246\|0	245\|0	135\|111	249\|0	250\|0	250\|0	246\|1
8	233\|0	240\|1	243\|0	250\|0	245\|0	242\|0	247\|0	248\|0	132\|113	240\|1	247\|0	241\|0
9	249\|0	246\|2	250\|0	250\|0	249\|0	220\|30	244\|0	249\|0	250\|0	249\|1	250\|0	249\|0
10	247\|0	177\|0	188\|0	119\|121	198\|0	244\|0	241\|0	176\|0	221\|0	224\|0	249\|0	174\|0
11	246\|1	172\|0	181\|0	208\|0	209\|0	198\|0	189\|0	175\|0	244\|0	182\|0	233\|0	95\|87
12	249\|1	195\|54	241\|0	249\|0	249\|0	249\|1	249\|0	250\|0	249\|0	250\|0	249\|1	250\|0
13	137\|0	91\|10	79\|0	131\|0	102\|0	103\|0	103\|0	83\|0	106\|0	111\|0	142\|0	90\|0