Aldehyde dehydrogenase activity helps identify a subpopulation of murine adipose-derived stem cells with enhanced adipogenic and osteogenic differentiation potential

Figure 2 Marker analysis of differentiation potentials. Differentiation potential of ALDHHi (A, C) and ALDHLo (B, D) subpopulations of ADSCs. FABP4 (A, B) and osteopontin (C, D) expression in ADSCs (red) following adipogenic (A, B) and osteogenic (C, D) differentiation of ALDH^Hi and ALDH^Lo subpopulations, as determined by immunocytochemistry. The nuclei were stained with Hoechst 33342 (blue). Quantitative analysis of differentiation-marker-positive areas in differentiated ADSCs. FABP4-positive (E) and osteopontin-positive (F) area ratios relative to respective areas of nuclear staining for ALDH^Hi and ALDH^Lo subpopulations of ADSCs after adipogenic (E) and osteogenic (F) induction. Values have been expressed in terms of mean ± SE (n = 5). ^aP < 0.05, ^bP < 0.0001, au: Arbitrary unit. ALDH: Aldehyde dehydrogenase; ADSCs: Adipose-derived stem cells; FABP: Fatty acid binding protein.