Endometrial mesenchymal stem cells as a cell based therapy for pelvic organ prolapse

Figure 6 Specific markers for ovine e-mesenchymal stem cells. Flow cytometry plot of ovine endometrial cells immunolabelled with CD271 and CD49f antibodies (A). The CD271⁺CD49f^- population enriches; Clonogenic stromal cells (B); Immunofluorescence images of ovine endometrium stained with CD271 (red) and vascular markers reveals their in vivo perivascular location in the adventitia of veins and arteries (C, D); vWF an endothelial marker (green), showing CD271⁺ cells are perivascular but not pericytes (C); αSMA, a perivascular marker (green) showing CD271⁺ cells located adjacent to αSMA⁺ cells in the adventitia of vessels rather than expressing αSMA themselves (D). White arrows: perivascular CD271⁺ cells; yellow arrows: CD271⁺ cells not associated with vessels (reproduced from ref.[81] with permission). vWF: Von Willebrand factor; αSMA: Alpha smooth muscle actin.