Endometrial mesenchymal stem cells as a cell based therapy for pelvic organ prolapse

Figure 5 Isolation and application of e-mesenchymal stem cells in pelvic organ prolapse vaginal repair. (A) simple office based endometrial biopsies can be used to obtain patients’ tissues, which are dissociated, then (B) eMSC selected using SUSD2 magnetic bead sorting, followed by (C) culture expansion in A83-01/serum free medium in 5% O₂ to generate large numbers of undifferentiated SUSD2⁺ eMSC (90%-95%) for (D) seeding onto fabricated scaffolds which will create an (E) eMSC/PA-G tissue engineering construct for implantation into (F) a large animal preclinical model to assess their efficacy in vaginal repair of parous ewes with evidence of POP (reproduced with permission from ref.[57,103] with permission). POP: Pelvic organ prolapse; MSC: Mesenchymal stem cells.