Mucosal-associated invariant T cells from induced pluripotent stem cells: A novel approach for modeling human diseases

Figure 2 Scheme for T cell differentiation from induced pluripotent stem cells. Induced pluripotent stem cells (iPSCs) derived from normal somatic cells such as fibroblasts possess the germline configuration at T cell receptor (TCR) loci, whereas those from T cells harbor rearranged configurations (A); Upon differentiation in the T-cell-permissive conditions, the resulting T cells possess diverse sets of TCR repertoires; polyclonal T cells. In contrast, mucosal-associated invariant T (MAIT) cells-derived iPSCs exclusively confer MAIT cells in the same differentiation conditions. Note that MAIT cell-derived iPSCs possess a rearranged Vα7.2-Jα-33 specific for MAIT cells in the genome (B).