Generation of diverse neural cell types through direct conversion

Figure 2 The process of neural direct conversion, as first described by Vierbuchen et al[16]. Somatic cells (A) are transduced with inducible lentiviral vectors expressing the neuronal transcription factors Brn2, Ascl1, and Myt1l (B), and cultured in a defined neuronal induction media (C). Following culture, induced neuronal cells can be identified by positive beta III tubulin staining (D), prior to further characterisation.