Use of platelet lysate for bone regeneration - are we ready for clinical translation?

Table 2 Summary of pre-clinical studies with platelet lysate-expanded bone marrow mesenchymal stem cells aimed at bone regeneration

Ref.	Animal model	MSC source	MSC expansion media (supplements)	Construct type	Scaffold pre-coating before loading with MSCs	Construct in vitro culture before implantation (supplements)
[38]	Mouse	Iliac crest BM	50 mL/L PL	BCP + 2 × 105 MSCs	A	10-8 M DEX
[36]	Sheep	Iliac crest BM	200 mL/L FCS	Rat-tail-derived collagen + 4 × 107 MSCs	PL	NR
[37]	Mouse	Iliac crest BM	100 mL/L FCS	HA (65%) + β-TCP (35%) + 3 × 105 MSCs	PL for 24 h	100 mL/L FCS
[25]1	Mouse	Iliac crest BM	50 mL/L PL	HA (65%) + β-TCP (35%) + MSCs2	PL for 24 h	50 mL/L PL
[35]1	Mouse	Iliac crest BM	50 mL/L PL	BCP + 4 × 106 MSCs	A	80 mL/L PL

¹No FCS was used at any stage of investigation;

²MSC number not reported. MSCs: Mesenchymal stem cells; BM: Bone marrow; PL: Platelet lysate; BCP: Biphasic calcium phosphate; A: Absent; DEX: Dexamethasone; FCS: Fetal calf serum; NR: Not reported; HA + β-TCP: Hydroxyapatite/beta-tricalcium phosphate.