Use of platelet lysate for bone regeneration - are we ready for clinical translation?

Table 1 Common methods of platelet lysate preparation

Ref.	Platelet collection	Leucocyte reduction	Freeze and thaw temperature (°C)	Anti-coagulation factors	PDGF level
[15]	Buffy coat	Inline filtration	-30/37	100 IU/mL H	(AA) 7424 ± 1043 pg/mL2
					(BB) 4655 ± 1353 pg/mL
					(AB) 40458 ± 1791 pg/mL
[18]	Buffy coat	Inline filtration	–30/37	2 IU/mL H	Higher than FCS
[38]1	Apheresis	NR	-20/37	NR	NR
[25]1	Apheresis	NR	-80/NR	2 IU/mL H	NR
[36]1	NR	NR	-80/NR	10 IU/mL H	NR
[51]	Apheresis	NR	-80/37	2 IU/mL H	(AB) 530 ± 60 pg/μL
[37]1	Apheresis	NR	-80/NR	NR	(AA) 19596 pg/mL
					(BB) 22329 pg/mL

¹Pre clinical investigation;

²Measured in media containing 10 mL/L platelet lysate. PDGF: Platelet derived growth factor; AA, AB and BB refer to different isotypes of PDGF; H: Heparin; FCS: Fetal calf serum; NR: Not reported.