Easily-handled method to isolate mesenchymal stem cells from coagulated human bone marrow samples

Figure 2 Colony-forming unit-fibroblast and mesenchymal stromal cell numbers of different groups. A: CFU-F numbers per 10⁶ nucleated cells from seven bone marrow samples; B-D: Bone marrow samples were diluted and split into 12 aliquots containing 10⁷ nucleated cells. The attached cells at passage 0 (B: × 10⁴), passage 1 (C: × 10⁵) and passage 2 (D: × 10⁵) were counted and summed up. The average and standard errors are shown. CFU-F: Colony-forming unit-fibroblast.