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Copyright ©The Author(s) 2015.
World J Stem Cells. Aug 26, 2015; 7(7): 1054-1063
Published online Aug 26, 2015. doi: 10.4252/wjsc.v7.i7.1054
Figure 1
Figure 1 Establishment of rat embryonic stem cells. A: E4.5 Blastocysts are isolated from Sprague-Dawley rats, and the blastocysts dissolved zona pellucida are put on mouse embryonic fibroblasts (MEF) feeders; B: After 7-10 d, the outgrowth formed from the blastocysts are dispersed, and transferred on MEF feeders; C: Approximately 7 d after culture, rat embryonic stem (ES) cells are appeared (our unpublished data).