Copyright
©The Author(s) 2015.
World J Stem Cells. Aug 26, 2015; 7(7): 1054-1063
Published online Aug 26, 2015. doi: 10.4252/wjsc.v7.i7.1054
Published online Aug 26, 2015. doi: 10.4252/wjsc.v7.i7.1054
Figure 1 Establishment of rat embryonic stem cells.
A: E4.5 Blastocysts are isolated from Sprague-Dawley rats, and the blastocysts dissolved zona pellucida are put on mouse embryonic fibroblasts (MEF) feeders; B: After 7-10 d, the outgrowth formed from the blastocysts are dispersed, and transferred on MEF feeders; C: Approximately 7 d after culture, rat embryonic stem (ES) cells are appeared (our unpublished data).
- Citation: Kawaharada K, Kawamata M, Ochiya T. Rat embryonic stem cells create new era in development of genetically manipulated rat models. World J Stem Cells 2015; 7(7): 1054-1063
- URL: https://www.wjgnet.com/1948-0210/full/v7/i7/1054.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v7.i7.1054