Adoptive immunotherapy for acute leukemia: New insights in chimeric antigen receptors

Figure 2 Schema of the transposon/transposase “sleeping beauty” system. A: The genetic cargo of a first plasmid (plasmid 1) is the chimeric antigen receptor (CAR) (anti-CD19 CD3ζ-CD28) flanked by inverted/short direct repeats (IR/DR). The whole set composed the transposon. The enzyme transposase is loaded in a second plasmid, which is specific of the IR/DR sequence; B: Materials of the two plasmids are fused together by electroporation, an electric current opening pores or channels at the cell surface. Transposase binds to the IR/DR sequences; C: The enzyme cut out of the plasmid the flanked sequences (CAR transgene with its promotor) and transfect the genetic cargo into a random DNA sequence of the recipient (T cell).