Simplified three-dimensional culture system for long-term expansion of embryonic stem cells

doi:10.4252/wjsc.v7.i7.1064

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Aug 26, 2015 (publication date) through Feb 15, 2025

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Aug 26, 2015; 7(7): 1064-1077
Published online Aug 26, 2015. doi: 10.4252/wjsc.v7.i7.1064

Table 3 Cell concentration for optimal growth of embryonic stem cells under 2-D and 3-D culture conditions

Culture conditions	Cell concentration
Culture conditions	(cells/mL)	(cells/cm²)
2-D Culture	---	3 × 10⁴ to 5 × 10^4[33]
3-D Culture	1 × 10⁴	1 × 10³

3-D: Three-dimensional; 2-D: Two-dimensional.

Citation: McKee C, Perez-Cruet M, Chavez F, Chaudhry GR. Simplified three-dimensional culture system for long-term expansion of embryonic stem cells. World J Stem Cells 2015; 7(7): 1064-1077
URL: https://www.wjgnet.com/1948-0210/full/v7/i7/1064.htm
DOI: https://dx.doi.org/10.4252/wjsc.v7.i7.1064