Utility of tricalcium phosphate and osteogenic matrix cell sheet constructs for bone defect reconstruction

Figure 3 X-ray images of constructs implanted into femurs. The femoral shaft was removed and replaced with BMSC/TCP constructs with or without cell sheets. Callus formation appeared around the BMSC/TCP constructs with cell sheets (BMSC/TCP/Sheet) at 2 wk post-implantation, was extended at 4 wk, and continued to the host bone at 8 wk. In contrast, the BMSC/TCP constructs showed non-union at 8 wk. Arrowheads indicate continuous bone formation. Bar = 2 mm. BMSCs: Bone marrow stromal cells; TCP: Tricalcium phosphate.