Copyright
©The Author(s) 2015.
World J Stem Cells. May 26, 2015; 7(4): 669-680
Published online May 26, 2015. doi: 10.4252/wjsc.v7.i4.669
Published online May 26, 2015. doi: 10.4252/wjsc.v7.i4.669
Trait | Embryonic stem cells | SSCs |
Mutagenesis technical status | Available for mice. Many genes mutated | Experimental level in mouse[75], rats[78], goats[81], pigs[82] |
Source | Embryo | Adult testis |
Age of derivation | Embryonic period | Postnatal period |
Ethical/legal concerns | Yes | No |
Efficiency | 5 × to 10 × higher than with ES cells | |
Cell differentiation state | Undifferentiated - pluripotential | Differentiated up to germ-line |
Tumorogenesis | Produce teratomas | Do not produce teratomas |
Chimera formation | (+) | (-) |
Germline gene transmission | Do not transmit genes from one generation to the next | Transmit genes from one generation to the next |
In vitro phenotype | Colonies with tightly attached cells | GS cells loosely attached (easily dissociated with trypsin)[2] |
Speed of growth | Faster | Slower |
Requires other non-transfected SSCs during culture | No | Yes2 |
Karyotype | Variable - unstable1 | Normal - stable |
Epigenetics | Variable - unstable DNA methylation pattern | Normal - stable DNA methylation pattern |
Offspring | Abnormal | Normal |
- Citation: Aponte PM. Spermatogonial stem cells: Current biotechnological advances in reproduction and regenerative medicine. World J Stem Cells 2015; 7(4): 669-680
- URL: https://www.wjgnet.com/1948-0210/full/v7/i4/669.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v7.i4.669