Niche interactions in epidermal stem cells

Figure 4 Immunohistochemical staining for involucrin, filaggrin, keratin 1, and α6 integrin in skin equivalents generated from fibroblasts or dermal sheath cells. Sections of skin equivalents (SEs) produced using fibroblasts (A, B, E, F, I, J, M, and N) or dermal sheath cells (C, D, G, H, K, L, O, and P) were stained for involucrin (A-D), filaggrin (E-H), keratin 1 (I-L), and α6 integrin (M-P). SEs were cultured in complete medium in the presence (B, D, F, H, J, L, N, and P) or absence (A, C, E, G, I, K, M, and O) of ascorbic acid. Expression of involucrin, filaggrin, keratin 1, and α6 integrin was up-regulated in SEs of dermal sheath cells compared with SEs of fibroblasts. Original magnification: 400 × (A-P).