Induction of CXC chemokines in human mesenchymal stem cells by stimulation with secreted frizzled-related proteins through non-canonical Wnt signaling

Figure 5 Effect of siRNA directed to frizzled receptors or receptor tyrosine kinase-like orphan receptor-2 on secreted frizzled-related protein-stimulated CXCL8 mRNA levels. sFRP-stimulated CXCL8 mRNA expression is inhibited in the presence of siRNAs directed toward non-canonical (A) Fzd2 and (B) Fzd5 or (C) the non-canonical Frizzled co-receptor RoR2. All values are mean ± SD. ^aP < 0.05 vs untreated siControl levels for each graph. sFRP1-3: Secreted frizzled-related protein 1-3; siFzd2/5: siRNA to the non-canonical frizzled receptor 2/5; RoR2: Receptor tyrosine kinase-like orphan receptor-2; siRoR2: siRNA to RoR2; siControl: Scrambled control siRNA.