Induction of CXC chemokines in human mesenchymal stem cells by stimulation with secreted frizzled-related proteins through non-canonical Wnt signaling

Figure 1 CXCL5 chemokine induction in human mesenchymal stem cells treated with osteogenic medium or non-canonical Wnt5a. A: CXCL5 mRNA levels are induced at 7 d in complete osteogenic medium or medium containing 0.1 μmol/L dexamethasone. Conditioned medium containing non-canonical Wnt5a induces (B) mRNA expression and (C) protein secretion. All values are mean ± SD. ^aP < 0.05; ^bP < 0.01 vs HMSCGM-treated group. HMSCGM: Human mesenchymal stem cell growth medium; DEX: Dexamethasone; OGM: Osteogenic medium; L-cell: Conditioned medium from L-cells; Wnt5a: Conditioned medium from L-cells expressing Wnt5a.