Dedifferentiated fat cells: A cell source for regenerative medicine

Figure 2 Schematic drawing of dedifferentiated fat cell preparation using preincubation and filters. Isolated adipocytes are incubated for 24 h in culture medium, before transfer to a new dish with filter, where the adipocytes remain for five days before the filter is removed. DFAT cells are allowed to sink through the filter to the bottom of the dish. The adipocytes and filter insert are then removed, and the new DFAT cells are cultured by regular methods. ASCs: Adipose-derived stem cells; DFAT: Dedifferentiated fat.