Early gestation chorionic villi-derived stromal cells for fetal tissue engineering

Figure 5 Placenta-derived mesenchymal stromal cells are compatible with biological and synthetic delivery vehicle matrices. A-D: Cell viability was assessed using Molecular Probes Live/Dead fluorescent assay kit for PMSCs cultured in various delivery vehicles. Results show that PMSCs were viable when culture in A: two-dimensional culture (control); B: 2 mg/mL collagen hydrogel; C: 5 mg/mL fibrin glue, D: an aligned PLLA/PLGA nanofiber scaffold. E-G: PMSCs can be efficiently transduced with Luciferase-GFP-containing lentiviral vector; E: phase contrast; F: green fluorescence images from the same field of view of transduced PMSCs; G: Flow cytometry analysis of transduced PMSCs indicate that they were transduced with an efficiency of 97.3%. Scale bars = 100 μm. PMSCs: Placenta-derived mesenchymal stromal cell.