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Copyright ©2014 Baishideng Publishing Group Inc.
World J Stem Cells. Jul 26, 2014; 6(3): 278-287
Published online Jul 26, 2014. doi: 10.4252/wjsc.v6.i3.278
Table 2 Animal studies for treatment of aortic aneurysmusing mesenchymal stem cells
Experimental AA modelCell sourceNumber of cellsInjection timeDeliveryEfficiencyRef.
ATII-infusion mouse modelBM-MSCCell-sheetSame time as ATII-infusionImplantation of MSC-sheet around infrarenal aorta4 wk after implantation, inhibition of AA development and growth, and elastin degradation downregulation of IL-1β, IL-6, MCP-1 and TNF-α protein expression, and MMP-2 and -9 enzymatic activity Up-regulation of IGF-1 and TIMP-1 protein expression Positive for MSC specific surface marker[51]
ATII-infusion mouse modelBM-MSC1 × 106/every week, 4 timesSame time as ATII-infusioniv-administration4 wk after injection, inhibition of AA development and growth, elastin degradation, M infiltration downregulation of IL-1β, IL-6 and MCP-1 protein expression, and MMP-2 and -9 enzymatic activity Up-regulation of IGF-1 and TIMP-1 protein expression Detection of MSC in the aortic wall[53]
Elastase-perfusion mouse modelPlacental-MSC1 × 1061 d after elastase-perfusioniv injection2 wk after injection, inhibition of AA expansion, inflammatory cell infiltration, and elastin degradation, downregulation of IL-17, IL-23, INF-γ, TNF-α, RANTES and MCP-1 protein expression Increase of α-SMA expression[54]
Xenograft rat modelBM-MSC1 × 106Same time as surgical interventionCatheter1 wk after surgical intervention inhibition of inflammatory cells infiltration and MMP-9 gene expression, and increase of TIMP-1 gene expression, after 4 wk, inhibition of AA expansion, increase of α-SMA expression, elastin and collagen content[56]
Dacron-patch pig modelASC1 × 106Same time as surgical interventionCatheterAttenuation of inflammation reaction, detection of ASC 3 wk after surgical intervention[57]
Balloon injury with type 1 collagen and elastase-perfusion porcine modelBM-MSC1 × 106Same time as balloon-injuryDirect injection into aortic wall72 h after injection, Increase of VEGF-A mRNA expression level 1 wk after injection, detection of GFP-labeled MSC at aortic wall and vWF positive cells formed tubuloluminal structures within outer layer of media and throughout the adventitia[54]