Embryonic stem cell-derived neural progenitors as non-tumorigenic source for dopaminergic neurons

Figure 3 Characterization of dopaminergic neurogenesis in human embryonic stem (H9) derived neural progenitor cells (HNP4) by expression analysis of marker genes. A: The expression of several specific marker genes (NGN2, NURR1, LMX1A, PITX3, TH and DAT) is displayed as determined by q-RTPCR analysis. The relative gene expression of differentiated neuronal cells (HNP neural diff) at passage 16 was normalized to the house-keeping gene GAPDH and the neural progenitors (HNP4). Three independent experiments were performed and the means + SE are indicated; B: The expression of several specific marker genes (NGN2, PAX6, PITX3, TUJ1, DAT and GFAP) was analyzed 30 d after starting neuronal differentiation of HNP4 cells at passages (p) 6, 9 and 16 by q-RTPCR analysis. The relative gene expression of differentiated neuronal cells was normalized to the house-keeping gene GAPDH and the neural progenitors (HNP4).